Lymphocyte receptors for polyclonal T mitogens: II. High-molecular-weight glycoproteins are the best candidate sites for mitogenic action by concanavalin A and galactose oxidase |
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Authors: | Jean J Favero Ian L Gordon John W Parker Richard L OBrien |
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Institution: | 1. Department of Pathology, University of Southern California School of Medicine, Los Angeles, California 90033 U.S.A.;2. Department of Pathology, University of Southern California Comprehensive Cancer Center, Los Angeles, California 90033 U.S.A. |
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Abstract: | Murine lymphocytes oxidized by galactose oxidase were radiolabeled by reduction with NaB3H4. The labeled cells were incubated with Con A and the Con A-Con A receptor complexes formed in situ on the viable cells were isolated by immuno-precipitation with anti-Con A serum and fixed Staphylococcus aureus. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis fluorography analysis of the precipitates demonstrated four high-molecular-weight glycoproteins which were oxidized by GO and which bound Con A. These same four glycoproteins were also oxidized and labeled by . 3H]Tyrosine biosynthetic labeling identified these four plus several other Con A receptors. Because Con A sterically inhibits GO mitogenic stimulation, these four glycoproteins are likely to represent the necessary sites of oxidative mitogenic action and are good candidates for the targets of Con A mitogenesis. |
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Keywords: | To whom all correspondence should be addressed at Department of Pathology USC School of Medicine 2025 Zonal Avenue Los Angeles Calif 90033 |
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