Lymphocyte receptors for polyclonal T mitogens: I. Concanavalin A binding to lymphocytes inhibits mitogenesis induced by galactose oxidase |
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Authors: | Ian L Gordon Paul H Coluzzi Jean J Favero John W Parker Richard L Obrien |
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Institution: | 1. Department of Pathology, University of Southern California School of Medicine, Los Angeles, California 90033 U.S.A.;2. Department of Pathology, University of Southern California Comprehensive Cancer Center, Los Angeles, California 90033 U.S.A. |
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Abstract: | Mitogenic stimulation of mouse lymphocytes by the enzyme galactose oxidase (GO) is inhibited if Con A is present during the enzymatic oxidation. The mechanism of this inhibition appears to involve steric hindrance of GO action at cell surface sites which bind Con A because (a) similar pulse exposure of unoxidized cells to Con A does not affect their subsequent ability to respond to GO stimulation; (b) Con A binding to fetuin interferes with GO oxidation of that glycoprotein substrate; and (c) sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of cells labeled by in the presence of Con A shows a selective inhibition of labeling of some high-molecular-weight glycoproteins compared to controls labeled in the absence of Con A. |
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Keywords: | To whom all correspondence should be addressed at Department of Pathology USC School of Medicine 2025 Zonal Avenue Los Angeles Calif 90033 |
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