A DNA primer/probe system for the rapid and sensitive detection of Mycobacterium tuberculosis-complex pathogens |
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| Authors: | Nicola T. Ralphs S. Garrett R. Morse J.B. Cookson P.W. Andrew G.J. Boulnois |
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| Affiliation: | Department of Microbiology, University of Leicester, Leicester, UK;Consultant Physician, Glenfield General Hospital, Leicester, UK |
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| Abstract: | A 1·5 kb Eco RI– Bam HI restriction fragment from Mycobacterium tuberculosis was found to hybridize specifically with genomic DNA from M. tuberculosis -complex organisms. Primers were designed from the terminal sequences of this fragment and used to amplify uniquely M. tuberculosis -group DNA in a polymerase chain reaction. It is suggested that a combination of these primers and probe will prove a useful tool for the early diagnosis of tuberculous infections. |
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