Decreased DNA damage by acid and increased repair of acid-damaged DNA in acid-habituated Escherichia coli |
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| Authors: | N Raja M Goodson D G Smith R J Rowbury |
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| Institution: | Biology Department, University College London, UK. |
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| Abstract: | A study of the conjugal transfer of ColV,I-K94 tn 10 from acid-treated donors suggested that acid-habituated recipients repair acid-damaged plasmid DNA better than those that are not habituated. The presence of an increased repair activity for acid-damaged DNA in habituated cells was confirmed by isolating pBR322 from acid-treated organisms; habituated cells produced more transformants when transformed by it than did non-habituated ones. Additionally, agarose gel electrophoretic studies of pBR322 DNA isolated from acid-damaged cells and tests of its transforming activity both indicated that plasmid DNA in habituated cells is less damaged by extreme acidity than is that in non-habituated organisms. |
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