Some effects of dibutylchloromethyltin chloride and other reagents on mitochondrial K+ flux |
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Authors: | Joyce Johnson Diwan |
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Institution: | (1) Biology Department, Rensselaer Polytechnic Institute, 12181 Troy, New York |
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Abstract: | Respiration-dependent K+ fluxes across the limiting membranes of isolated rat liver mitochondria, measured by means of42K, are stimulated by the oxidative phosphorylation inhibitor dibutylchloromethyltin chloride (DBCT). A lack of effect of Cl– concentration indicates that the stimulation of K+ flux by DBCT is not attributable to Cl–/OH– exchange activity. The mercurial mersalyl was previously shown to stimulate respiration-dependent K+ influx. The combined presence of mersalyl plus DBCT results in a greater stimulation of K+ influx than is caused by either DBCT or mersalyl alone. The oxidative phosphorylation inhibitor oligomycin, which alone has no effect on respiration-dependent K+ influx, enhances the stimulatory effect of mersalyl on K+ influx. The data are consistent with, although not proof of, a direct interaction of the K+ transport mechanism with the mitochondrial energy transduction apparatus.Abbreviations used: DCCD,N,N -dicyclohexylcarbodiimide; DBCT, dibutylchloromethyltin chloride. |
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Keywords: | Mitochondria DBCT K+ transport mersalyl |
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