Quorum sensing system lactones do not increase invasiveness of a MexAB-OprM efflux mutant but do play a partial role in Pseudomonas aeruginosa invasion |
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| Authors: | Kondo Akira Hirakata Yoichi Gotoh Naomasa Fukushima Kazuko Yanagihara Katsunori Ohno Hideaki Higashiyama Yasuhito Miyazaki Yoshitugu Nishide Kiyoharu Node Manabu Yamada Yasuaki Kohno Shigeru Kamihira Shimeru |
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| Affiliation: | Department of Laboratory Medicine, Nagasaki University School of Medicine and Dentistry, Nagasaki, Nagasaki 852-850, Japan. |
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| Abstract: | We studied the quorum sensing (QS) system and the related homoserine lactones (HSLs) observing Pseudomonas aeruginosa invasion using the epithelial cell monolayer penetration assay model. Compared to the PAO1 wild-type, the QS mutants, DeltalasI and DeltarhlI, were compromised in their capacity to invade. The decreased invasiveness of DeltarhlI was restored by adding 100 microM exogenous C(4)-HSL. However, the decreased invasiveness of an efflux mutant, DeltamexAB-oprM, was not restored in the presence of exogenous HSLs. The QS system partially plays a role in P. aeruginosa invasion; however, C(4)-HSL and 3-O-C(12)-HSL are not the essential determinants for invasiveness for P. aeruginosa. |
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| Keywords: | pseudomonas aeruginosa quorum sensing efflux invasion |
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