The Quantitative Determination of Osmium Tetroxide in Fixatives |
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Authors: | Norman A Frigerio B R Nebel |
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Institution: |
a Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois |
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Abstract: | This rapid spectrophotometric method for determining the OsO4 concentration in fixative and stock solutions is based on the reduction of OsO4 by acidified KI to the blue species of OsI6 =, which is then determined at 649 mµ. The salt K2OsI6 has been isolated from the reaction mixture and characterized. Method: A I ml aliquot of the solution, containing up to 3% OsO4, is diluted to 100 ml with distilled water. To 1 ml of the diluted solution is added, in order: distilled water, 2 ml; 1 M HCI, 1 ml; and 1 M KI, 1 ml. Optical density at 649 mµ is read from 10-120 min thereafter. OsO4 concentration is calculated from the measured molecular extinction coefficient of OsI6 =, 4400 liter/mole cm. |
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