A chimeric baculovirus displaying bovine herpesvirus-1 (BHV-1) glycoprotein D on its surface and their immunological properties |
| |
Authors: | A Peralta P Molinari D Conte-Grand G Calamante O Taboga |
| |
Institution: | (1) Instituto de Biotecnología, CICVyA, INTA, Castelar, CC25 (1712), Buenos Aires, Argentina;(2) Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Rivadavia, 1917 (1033), Ciudad Autónoma de Buenos Aires, Argentina |
| |
Abstract: | The ability of a recombinant baculovirus containing the ectodomain of the mature sequence of glycoprotein D (gD) fused to
the amino-terminus of baculoviral glycoprotein gp64 to display gD on its surface and to serve as an improved immunogen against
bovine herpesvirus-1 was tested. The gD–gp64 fusion protein was correctly expressed on the virus particles as revealed by
immunomicroscopy assays. Mice immunized with 5 × 108 plaque forming units developed antibodies that specifically reacted in an enzyme-linked immunosorbent assay with recombinant
gD and whole bovine herpesvirus-1. These antibodies were able to neutralize bovine herpesvirus-1 in vitro, whereas those elicited
by a version of gD expressed in Escherichia coli did not. Our data demonstrated that the display on the virion surface of recombinant baculovirus can provide a tool for the
development of recombinant vaccines against bovine herpesvirus-1. |
| |
Keywords: | Baculovirus display BHV-1 vaccines Viral vectors |
本文献已被 PubMed SpringerLink 等数据库收录! |
|