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Fusion of bacteriorhodopsin with submitochondrial particles yields a new system with retention of energy coupling and acquisition of photophosphorylation activity |
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| Authors: | Serena Seren Rita Casadio M.Catia Sorgato |
| Affiliation: | a Istituto di Chimica Biologica e Centro Studio Fisiologia Mitocondriale, C.N.R., Via F. Marzolo, 3, 35131, Padova, Italy b Istituto ed Orto Botanico dell'Università, 40126, Bologna, Italy |
| Abstract: | Submitochondrial particles were fused with purple membranes of Halobacterium halobium cells by means of a freeze-thawe sonication procedure. It is reported that fusion of inner mitochondrial membranes with a bacterial membrane yields a new particle which shows not only retention of redox- and photon-linked energy-coupling activities, but also creation of an additional energy-coupling process, light-driven ATP synthesis. |
| Keywords: | Bacteriorhodopsin Purple membrane Mitochondrial membrane Membrane fusion Photophosphorylation (Bovine heart, Submitochondrial particle) |
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