| 固定化胰蛋白酶活力的测定方法探讨 |
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| 引用本文： | 库汉生,姚晓玲,张婷,刘丹松. 固定化胰蛋白酶活力的测定方法探讨[J]. 氨基酸和生物资源, 2007, 29(2): 33-35. DOI: 10.3969/j.issn.1006-8376.2007.02.011 |
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| 作者姓名： | 库汉生 姚晓玲 张婷 刘丹松 |
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| 作者单位： | 湖北工业大学生物工程学院,湖北,武汉,430068;湖北工业大学生物工程学院,湖北,武汉,430068;湖北工业大学生物工程学院,湖北,武汉,430068;湖北工业大学生物工程学院,湖北,武汉,430068 |
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| 基金项目： | 湖北省教育厅科学技术研究项目(项目编号2004D003) |
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| 摘 要： | 对自制的固定化胰蛋白酶(Immobilization Trypsin,ITP)分别经过冷冻干燥、真空干燥后定量测定其酶活力,与由湿态直接定量法(DQA)测定的活力进行比较。试验表明,直接定量方法测定的酶活力比前两者高,而且操作简单。
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| 关 键 词： | 固定化 胰蛋白酶 活力回收率 |
| 文章编号： | 1006-8376（2007）02-0033-03 |
| 修稿时间： | 2007-01-22 |
Study on Determination of the Activity of Immobilized Trypsin |
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| KU Han-sheng,YAO Xiao-ling,ZHANG Ting,LIU Dan-song. Study on Determination of the Activity of Immobilized Trypsin[J]. Amino Acids & Biotic Resources, 2007, 29(2): 33-35. DOI: 10.3969/j.issn.1006-8376.2007.02.011 |
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| Authors: | KU Han-sheng YAO Xiao-ling ZHANG Ting LIU Dan-song |
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| Affiliation: | School of Bioengineering, Hubei University of Technology , Wuhan 430068, China |
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| Abstract: | The directly quantitative analysis(DQA) methods to measure the activity of the immobilized trypsin were studied,and were compared to measurement on the activity of the immobilized trypsin dried by freeze or vacuum. The results showed that the activity on DQA was higher than that on the others. |
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| Keywords: | immobilization trypsin activity |
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