TRPC3 channels are required for synaptic transmission and motor coordination |
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Authors: | Hartmann Jana Dragicevic Elena Adelsberger Helmuth Henning Horst A Sumser Martin Abramowitz Joel Blum Robert Dietrich Alexander Freichel Marc Flockerzi Veit Birnbaumer Lutz Konnerth Arthur |
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Institution: | Institute of Neuroscience and Center for Integrated Protein Science, Technical University Munich, 80802 Munich, Germany. |
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Abstract: | In the mammalian central nervous system, slow synaptic excitation involves the activation of metabotropic glutamate receptors (mGluRs). It has been proposed that C1-type transient receptor potential (TRPC1) channels underlie this synaptic excitation, but our analysis of TRPC1-deficient mice does not support this hypothesis. Here, we show unambiguously that it is TRPC3 that is needed for mGluR-dependent synaptic signaling in mouse cerebellar Purkinje cells. TRPC3 is the most abundantly expressed TRPC subunit in Purkinje cells. In mutant mice lacking TRPC3, both slow synaptic potentials and mGluR-mediated inward currents are completely absent, while the synaptically mediated Ca2+ release signals from intracellular stores are unchanged. Importantly, TRPC3 knockout mice exhibit an impaired walking behavior. Taken together, our results establish TRPC3 as a new type of postsynaptic channel that mediates mGluR-dependent synaptic transmission in cerebellar Purkinje cells and is crucial for motor coordination. |
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