Trypanosoma rhodesiense: mitochondrial proteins of bloodstream and procyclic trypomastigotes |
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| Authors: | R B Le Febvre G C Hill |
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| Affiliation: | 1. Life Science Research Centre, Faculty of Science, University of Ostrava, 710 00 Ostrava, Czechia;2. Institute of Parasitology, Czech Academy of Sciences, 370 05 České Budějovice, Czechia;3. Faculty of Sciences, University of South Bohemia, 370 05 České Budějovice, Czechia;4. Faculty of Biology, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia;1. Department of Chemistry, Biochemistry, and Pharmaceutical Sciences, University of Bern, Freiestrasse 3, Bern CH-3012, Switzerland;2. Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern CH-3012, Switzerland;3. Institute for Advanced Study (Wissenschaftskolleg) Berlin, D-14193 Berlin, Germany;1. Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic;2. Faculty of Sciences, University of South Bohemia, České Budějovice (Budweis), Czech Republic;3. Medical Biochemistry, University of Amsterdam, AMC, Amsterdam, The Netherlands;4. Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI, USA;5. School of Life Sciences, University of Dundee, Dundee, UK |
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| Abstract: | One- and two-dimensional gel electrophoresis of the solubilized mitochondrial proteins of bloodstream and procyclic trypomastigote Trypanosoma brucei rhodesiense and radiolabeling of proteins in the presence of cycloheximide were used to identify proteins synthesized in the trypanosome mitochondrion. The proteins which comprise the mitochondrion were found to be very similar in both bloodstream and procyclic trypomastigotes, but do differ in their level of synthesis. A protein putatively identified as subunit II of cytochrome oxidase (EC 1.9.3.1) was detected in mitochondria from both the procyclic and bloodstream organisms. The presence of this protein in bloodstream trypomastigotes and the overall similarity of protein content in the trypanosome mitochondria is noteworthy in view of the fact that bloodstream trypomastigotes have a repressed mitochondrion with no detectable tricarboxylic acid cycle or cytochrome electron transport chain. |
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