Perchlorate and nitrate reductase activity in the perchlorate-respiring bacterium perclace |
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Authors: | T Giblin WT Frankenberger |
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Institution: | aDepartment of Environmental Sciences, 2217 Geology Bldg., University of California, Riverside, CA 92521, USA |
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Abstract: | The perchlorate (ClO4?)-respiring organism, strain perc1ace, can grow using nitrate (NO3?) as a terminal electron acceptor. In resting cell suspensions, NO3? grown cells reduced ClO4?, and ClO4? grown cells reduced NO3?. Activity assays showed that nitrate reductase (NR) activity was 1.31 μmol min?1 (mg protein)?1 in ClO4? grown cells, and perchlorate reductase (PR) activity was 4.24 μmol min?1 (mg protein)?1 in NO3? grown cells. PR activity was detected within the periplasmic space, with activities as high as 14 μmol min?1 (mg protein)?1. The NR had a pH optimum of 9.0 while the PR had an optimum of 8.0. This study suggests that separate terminal reductases are present in strain perclace to reduce NO3? and ClO4?. |
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Keywords: | perchlorate reduction nitrate reduction perclace groundwater |
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