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Repeated-batch culture of immobilizedGibberella fujikuroi B9 for gibberellic acid production: An optimization study |
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| Authors: | Chang-Joon Kim Sang Jong Lee Yong Keun Chang Gie-Taek Chun Yeon-Ho Jeong Sung Bae Kim |
| Institution: | 1. Department of Chemical and Biological Engineering and ERI, Gyeongsang National University, 660-701, Jin Ju, Korea 2. STR Biotech. Co., Ltd., 200-161, Chunchon, Korea 3. Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 305-701, Daejeon, Korea 4. Department of Molecular Bioscience, Kangwon National University, 200-701, Chunchon, Korea 5. Department of Molecular and Medical Biotechnology, Kangwon National University, 200-701, Chunchon, Korea |
| Abstract: | The performance of immobilized fungal cells on celite beads for the production of gibberrelic acid was investigated in flasks and 7-L stirred-tank reactor. Repeated incubations of immobilized fungal cells increased cell concentrations and volumetric productivity. The maximum volumetric productivity obtained in the immobilized-cell culture was 3-fold greater than that in suspended-cell culture. The concentration of cotton seed flour (CSF), amont the various nutrients supplied, most significantly influenced productivity and operational stability. Notably, insoluble components in CSF were found to be essential for production. CSF at 6 g/L with 60 g/L glucose was found to be optimal for gibberellic acid production and stable operation by preventing excessive cell growth. |
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