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Minicircle Performance Depending on S/MAR-Nuclear Matrix Interactions

Authors:	Sandra Broll Kathrin Hahn Juergen Bode

Institution:	¹ Department Molecular Biotechnology/Epigenetic Regulation, Helmholtz Center for Infection Research, Inhoffenstraße 7, D-38124 Braunschweig, Germany ² Hannover Medical School (MHH, Hans-Borst Center for Heart and Stem Cell Research), OE6960-Experimental Hematology, Carl-Neuberg-Strasse 1, D-30625 Hannover, Germany ³ RWTH Aachen, Biotechnology/Molecular Biotechnology, Aachen, Germany

Abstract:

Keywords:	BPV bovine papillomavirus BT butyrate treatment BUR DNA base-unpairing region CB confluence block CHO Chinese hamster ovary CUE core-unpairing element EBV Epstein-Barr virus eGFP enhanced green fluorescent protein FACS fluorescence-activated cell sorting FCS fetal calf serum FISH fluorescence in situ hybridization HDACi histone deacetylase inhibitor IR initiator of replication MC minicircle MP miniplasmid ori origin of replication oriP plasmid origin of replication ORC origin recognition complex PBS phosphate-buffered saline PD population doubling PP parental plasmid = educt for MC preparation pre-RC pre-replication complex S/MAR scaffold/matrix attachment region SIDD stress-induced duplex destabilization SV40 simian virus 40 UE DNA unpairing element
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