Light Activation of Rhodopsin: Insights from Molecular Dynamics Simulations Guided by Solid-State NMR Distance Restraints |
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Authors: | Viktor Hornak Markus Eilers Mordechai Sheves Steven O. Smith |
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Affiliation: | 1 Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794-5215, USA 2 Department of Physics and Astronomy, Stony Brook University, Stony Brook, NY 11794-5215, USA 3 Department of Organic Chemistry, Weizmann Institute, Rehovot, Israel 4 Department of Biological Sciences, University of Essex, Wivenhoe Park, Essex C04 3SQ, UK |
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Abstract: | Structural restraints provided by solid-state NMR measurements of the metarhodopsin II intermediate are combined with molecular dynamics simulations to help visualize structural changes in the light activation of rhodopsin. Since the timescale for the formation of the metarhodopsin II intermediate (> 1 ms) is beyond that readily accessible by molecular dynamics, we use NMR distance restraints derived from 13C dipolar recoupling measurements to guide the simulations. The simulations yield a working model for how photoisomerization of the 11-cis retinylidene chromophore bound within the interior of rhodopsin is coupled to transmembrane helix motion and receptor activation. The mechanism of activation that emerges is that multiple switches on the extracellular (or intradiscal) side of rhodopsin trigger structural changes that converge to disrupt the ionic lock between helices H3 and H6 on the intracellular side of the receptor. |
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Keywords: | GPCR, G-protein-coupled receptor TM, transmembrane Meta I, metarhodopsin I Meta II, metarhodopsin II SB, Schiff base PSB, protonated Schiff base MD, molecular dynamics PDB, Protein Data Bank EL2, second extracellular loop FTIR, Fourier transform infrared spectroscopy MAS, magic angle spinning DARR, dipolar-assisted rotational resonance |
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