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The spatial organization of nucleolar DNA in phytohemagglutinin-stimulated lymphocytes of the guinea pig |
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| Authors: | Dr. A. Anteunis M. Pouchelet A. Gansmüller R. Robineaux |
| Affiliation: | (1) Centre de Physiologie et d'Immunologie Cellulaires, INSERM U.104, C.N.R.S. et Association Claude Bernard, Hôpital Saint-Antoine, Paris, France;(2) INSERM U.104, Hôpital Saint-Antoine, 184, rue du Fbg St-Antoine, 75571 Paris Cedex 12, France |
| Abstract: | Summary The ultrastructural changes in the spatial organization of nucleolar DNA in lymphocytes during phytohemagglutinin (PHA) stimulation was studied in guinea pigs by means of oxidized diaminobenzidine (DAB) at low pH as a differentially contrasting stain for nucleic acids and by the use of reconstruction of serial sections.The extended DNA filaments situated inside the fibrillar area originate from a large aggregation of heterochromatin, which is closely associated with the nucleolus, and from the perinucleolar shell of condensed chromatin. It is suggested that these two distinct regions of chromatin might be associated with different functions. |
| Keywords: | Lymphocytes Phytohemagglutinin stimulation Nucleolar organizer region Three-dimensional reconstruction Ultrastructure Guinea pig |
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