Biobleaching of wheat straw pulp with recombinant laccase from the hyperthermophilic <Emphasis Type="Italic">Thermus thermophilus</Emphasis> |
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| Authors: | Zhiqiang Zheng Huazhong Li Lun Li Weilan Shao |
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| Institution: | (1) Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, People’s Republic of China;(2) Faculty of Veterinary Science, University of Sydney, Camden, NSW, Australia;(3) Research Center for Biotechnology and Biomass Energy, Nanjing Normal University, Nanjing, 210046, People’s Republic of China;(4) Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing, 210046, People’s Republic of China; |
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| Abstract: | The recombinant laccase from Thermus thermophilus was applied to the biobleaching of wheat straw pulp. The best bleaching effect was when the pulp was treated with 3 U laccase g−1 dry pulp at 90°C, pH 4.5, 8% consistency for 1.5 h. Under these conditions, the pulp brightness was increased by 3.3% ISO,
and the pulp kappa number was decreased by 5.6 U. Enzymatic treatment improved the bleachability of wheat straw pulp but caused
no damage to the pulp fibers. The use of enzyme-treated pulp saved 25% H2O2 consumption in subsequent peroxide bleaching without decreasing the final brightness. Pulp biobleaching in the presence of
5 mM ABTS further increased the pulp brightness by 1.5% ISO. This is the first report on the application of laccase from T. thermophilus in the pulp and paper sector. |
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