Isolation and functional characterization of polyunsaturated fatty acid elongase (AsELOVL5) gene from black seabream (Acanthopagrus schlegelii) |
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Authors: | Sun Hee Kim Jong Bum Kim Yo Soon Jang So Yun Kim Kyung Hee Roh Hyun Uk Kim Kyeong-Ryeol Lee Jong-Sug Park |
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Institution: | (1) National Academy of Agricultural Science, Rural Development Administration, Seodun-Dong, Suwon, 441-707, Korea;(2) East Sea Environment Research Department, KORDI, 695-1, Hujung-ri, Jukbyeon-myun, Uljin-gun, Gyeongbuk, Seoul, 767-813, Korea |
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Abstract: | To identify the genes encoding fatty acid elongases for the biosynthesis of polyunsaturated fatty acids (PUFAs), we isolated
a cDNA via degenerate PCR and RACE-PCR from Acanthopagrus schlegelii with a high similarity to the ELOVL5-like elongases of mammals and fishes. This gene is termed AsELOVL5 and encodes a 294 amino acid protein. When AsELOVL5 was expressed in Saccharomyces cerevisiae, it conferred an ability to elongate γ-linolenic acid (18:3 n−6) to di-homo-γ-linolenic acid (20:3 n−6). In addition, the
transformed cells converted arachidonic acid (20:4 n−6) and eicosapentaenpic acid (20:5 n−3) to docosatetraenoic acid (22:4
n−6) and docosapentaenoic acid (22:5 n−3), respectively. These results indicate that the AsELOVL5 gene encodes a long-chain fatty acid elongase capable of elongating C18Δ6/C20Δ5 but not C22 PUFA substrates. |
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