Analysis of replication factories in human cells by super-resolution light microscopy |
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Authors: | Zoltan Cseresnyes Ulf Schwarz Catherine M Green |
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Affiliation: | (1) Department of Zoology, University of Cambridge, Downing Street, Cambridge, CB2 3EJ, UK;(2) Leica Microsystems, CMS GmbH, Am Friedensplatz 3, 68165 Mannheim, Germany |
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Abstract: | Background DNA replication in human cells is performed in discrete sub-nuclear locations known as replication foci or factories. These factories form in the nucleus during S phase and are sites of DNA synthesis and high local concentrations of enzymes required for chromatin replication. Why these structures are required, and how they are organised internally has yet to be identified. It has been difficult to analyse the structure of these factories as they are small in size and thus below the resolution limit of the standard confocal microscope. We have used stimulated emission depletion (STED) microscopy, which improves on the resolving power of the confocal microscope, to probe the structure of these factories at sub-diffraction limit resolution. |
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