Detection of two forms of glutamine synthetase in Bacillus brevis (A. G. 4) |
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Authors: | N K Gaur A Kumar G K Garg |
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Institution: | Department of Biochemistry G.B.Pant University of Agriculture and Technology Pantnagar, Distt. Nainital. India 263 145 |
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Abstract: | A laboratory isolate of could grow and sporulate on an amino acid, viz., alanine or glutamate or aspartate as single source of carbon and nitrogen. It failed to sporulate if the amino acid was replaced by the corresponding keto acid and ammonium sulphate in the medium, although, normal growth was observed. One of the key enzymes in nitrogen assimilation, the glutamine synthetase, has been purified by DE-52 and affinity column chromatography from both alanine and pyruvate grown cells. The kinetic and other properties of both of these enzymes were studied. The enzyme isolated from alanine grown cells differed significantly from that isolated from pyruvate grown cells (viz.,pH optima, response to Mg++ and other effectors). A possible role of glutamine synthetase in the initiation of bacterial sporulation is discussed. |
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