| 斑马鱼胚胎显微注射基因分析平台的建立 |
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| 引用本文： | 陶大昌,陈惠娟,赵君,马用信. 斑马鱼胚胎显微注射基因分析平台的建立[J]. 四川动物, 2012, 31(3): 468-470,513 |
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| 作者姓名： | 陶大昌 陈惠娟 赵君 马用信 |
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| 作者单位： | 四川大学华西医院医学遗传研究室,成都,610041 |
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| 摘 要： | 斑马鱼作为一种新的理想模式动物,已在发育生物学、环境毒理学和人类疾病及相关基因功能等研究中得到广泛应用。本文就建立斑马鱼胚胎的显微注射基因分析平台进行了探究,并通过piwil2基因的抑制和过表达实验验证了平台的可操作性,也对建立平台中所要注意的问题进行了讨论。
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| 关 键 词： | 斑马鱼 显微注射 piwil2 |
Platform for the Microinjection and Genetic Analysis of Zebrafish Embryos |
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| TAO Da-chang , CHEN Hui-juan , ZHAO Jun , MA Yong-xin. Platform for the Microinjection and Genetic Analysis of Zebrafish Embryos[J]. Sichuan Journal of Zoology, 2012, 31(3): 468-470,513 |
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| Authors: | TAO Da-chang CHEN Hui-juan ZHAO Jun MA Yong-xin |
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| Affiliation: | * (Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu 610041, China) |
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| Abstract: | Zebrafish, an ideal model animal, has been widely used in developmental biology, environmental toxicology and functional analysis of human disease-related genes. In this study, we built a microinjection platform of zebrafish embryos and demonstrated the workability of this platform by knockdown and over-expression of zebrafish piwil2 gene. Moreover, we also discussed the problems of platform structuring. |
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| Keywords: | zebrafish microinjection piwil2 |
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