Mung Bean Hypocotyl Homogalacturonan: Localization, Organization and Origin |
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| Authors: | LIBERMAN M; MUTAFTSCHIEV S; JAUNEAU A; VIAN B; CATESSON A M; GOLDBERG R |
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| Institution: | Laboratoire de Biologie Cellulaire Végétale, Université P. et M. Curie, Paris Laboratoire de Physiologie Végétale Appliquée, Université P. et M. Curie, Paris Laboratoire d'Enzymologie en milieu structuré, Institut J. Monod, Paris S.C.U.E.O.R., Université de Rouen Laboratoire de Pathologie Végétale, Institut National Agronomique, , Paris-Grignon |
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| Abstract: | Specific antibodies were used to localize both pectic structuresand pectinmethylesterases (PME) along the mung bean hypocotyl.Calcium ions were also detected and estimated in both young,plastic and mature, stiffened cell walls. Highly methylesterifiedpectins were present in all cell walls but decreased from thehypocotyl hook downwards. Expanded cell walls were characterizedby a high content of calcium ions and acidic pectins, althoughthe latter's cross-reactivity to JIM 5 antibodies was partlylost. Co-localization of acidic homogalacturonan and calciumions suggests the presence of egg-box structures that mightparticipate in the cell wall stiffening process which developsalong the hypocotyl. Acidic polymers could originate from theactivity of the pectinmethylesterases present in precise wallareas but direct export of acidic polygalacturonan through Golgivesicles was also observed. Copyright 1999 Annals of BotanyCompany Cell walls, immunolocalization, hypocotyl, mung bean, pectin organization, Vigna radiata. |
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