Influence of precursors and additives on microbial lipases stabilized by sol-gel entrapment |
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| Authors: | Francisc P ter L szl Poppe Claudia Kiss Emese Szocs-Bí r Gabriela Preda Cristina Zarcula Alina Olteanu |
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| Institution: |
a Department of Organic Chemistry and Technologies, Faculty of Industrial Chemistry and Environmental Engineering, “Politehnica” University of Timisoara, Timisoara, Romania
b Institute for Organic Chemistry and Research Group for Alkaloid Chemistry of the Hungarian Academy of Sciences, Budapest University of Technology and Economics, Budapest, Hungary
c Department of Chemistry, Faculty of Chemistry-Biology-Geography, West University of Timisoara, Timisoara, Romania |
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| Abstract: | Sol-gel entrapment of microbial lipases from Candida cylindracea (Cc lipase), Pseudomonas fluorescens (Lipase AK), and Pseudomonas cepacia (Lipase PS), using as precursors tetraethoxysilane (TEOS) and silanes of type R-Si(OEt)3 with alkyl or aryl R groups, has been investigated. Three different methods using these precursors were tried exhibiting protein immobilization yields in the range of 20-50%. Hydrolysis of emulsified olive oil, esterification of lauric acid with 1-octanol and enantioselective acylation of 2-pentanol have been used as model reactions for testing the properties of the encapsulated lipases. The recovery yields of the enzyme activity in the esterification reaction were between 20-68%, the best performance being achieved with phenyltriethoxysilane and tetraethoxysilane precursors at 3:1 molar ratio. When testing the entrapped Lipase AK in the enantioselective acylation reaction of 2-pentanol, activity recovery yields up to 32% related to the free enzyme were obtained and the immobilization increased the enantioselectivity of the enzyme. |
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| Keywords: | Enantioselectivity microbial lipase silane precursor sol-gel entrapment |
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