| 变性梯度凝胶电泳在堆肥微生物研究中的应用 |
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| 引用本文： | 崔宗均,宫小燕,李国学. 变性梯度凝胶电泳在堆肥微生物研究中的应用[J]. 微生物学通报, 2004, 31(5): 116-119 |
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| 作者姓名： | 崔宗均 宫小燕 李国学 |
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| 作者单位： | 1. 中国农业大学农业与生物技术学院,北京,100094
2. 中国农业大学资源与环境学院,北京,100094 |
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| 基金项目： | 国家“十五”科技攻关计划重大项目 (No 2 0 0 2BA5 1 6A0 3 ) |
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| 摘 要： | 对当前堆肥中微生物种群分布及其对有机物分解作用的研究进行分析，论述了分子生物技术中的变性梯度凝胶电泳(DGGE)的特点。将DGCE与PCR扩增技术相结合，可用于研究自然菌种堆肥和人工培养驯化菌种堆肥过程中微生物的演替规律，为研究和筛选堆肥中的微生物提供更加有效、快速的信息，促进堆肥技术的发展。
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| 关 键 词： | 变性梯度凝胶电泳 堆肥 微生物演替 |
| 文章编号： | 0253-2654(2004)05-0116-04 |
| 修稿时间： | 2003-11-17 |
Application of Denaturing Gradient Gel Electrophoresis on Sturdy of Microbe in Composting Process |
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| CUI Zong-Jun GONG Xiao-Yan LI Guo-Xue. Application of Denaturing Gradient Gel Electrophoresis on Sturdy of Microbe in Composting Process[J]. Microbiology China, 2004, 31(5): 116-119 |
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| Authors: | CUI Zong-Jun GONG Xiao-Yan LI Guo-Xue |
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| Affiliation: | CUI Zong-Jun1 GONG Xiao-Yan 2** LI Guo-Xue1 |
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| Abstract: | Studies on the distribution of microbe and their degradation of organic material in compost are presented, and the character of denaturing gradient gel electrophoresis (DGGE) method is also discussed. Combined with PCR technology, DGGE could be effectively applied to investigate microbial succession in composting with natural microorganism and with bred microbial community, which could provide prompt and efficient information to facilitate development of composting technology. |
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| Keywords: | DGGE Compost Microbial succession |
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