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| 菌物DNA条形码技术原理与操作 | |
| 引用本文： | 刘淑艳,张傲,李玉.菌物DNA条形码技术原理与操作[J].菌物研究,2012,10(3):205-209. |
| 作者姓名： | 刘淑艳 张傲 李玉 |
| 作者单位： | 1. 食药用菌教育部工程研究中心,长春130118／吉林农业大学农学院,长春130118 2. 吉林农业大学农学院,长春,130118 3. 食药用菌教育部工程研究中心,长春,130118 |
| 基金项目： | 国家自然科学基金项目(30970017,31170013);教育部博士基金项目(20102223120003);吉林省教育厅项目[(2009)358号] |
| 摘 要： | DNA条形码技术是通过对1个较短目的基因的DNA序列进行分析从而进行物种鉴定的方法,它通过对1个或多个相关基因进行大范围的扫描,进而鉴定未知物种或者发现新种。当传统的分类学受到阻碍时,这种技术可以发挥其优势。相对于其他生物,菌物的生活史独特而复杂,这就使得对其进行的形态学鉴定要受到菌物自身生长发育时期的限制。国内外科学家对寻找适合于大多数菌物的标准DNA条码进行过探索,但还没有找到满足全部特征的基因片段。文中对DNA条形码技术的概念、原理依据、操作步骤和优缺点方面进行了介绍,并对DNA条形码技术在我国菌物研究方面的应用前景进行了展望。 |
| 关 键 词： | 菌物 基因条形码 操作步骤 应用前景 |
Principle and Procedure of Fungal DNA Barcoding |
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| LIU Shu-yan,ZHANG Ao,LI Yu.Principle and Procedure of Fungal DNA Barcoding[J].Journal of Fungal Research,2012,10(3):205-209. | |
| Authors: | LIU Shu-yan ZHANG Ao LI Yu |
| Institution: | 1(1.Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Changchun 130118,China;2.College of Agronomy,Jilin Agricultural University,Changchun 130118,China) |
| Abstract: | DNA barcode is a technology which uses DNA sequence as a system to accomplish identification.It can be used to indentify unknown species and discover new species by scanning and analyzing DNA fragments.The advantages of this technology stand out when traditional taxonomy is challenged by life-stage transformations of their specimen,especially in fungi.Many scientists have been working on selecting a suitable gene which should be used as the standard DNA barcode for fungi.The concept,principle,procedures,advantages and disadvantages of DNA barcode were discussed in the present paper.The prospect for this technology was briefly described. |
| Keywords: | fungi gene barcode procedure prospect for application |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |