Immobilization of tyrosinase for use in nonaqueous media: Enzyme deactivation phenomena |
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| Authors: | Stephen H Day Raymond L Legge |
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| Institution: | (1) Biochemical Engineering Group, Department of Chemical Engineering, University of Waterloo, N2L 3G1 Waterloo, Ontario, Canada |
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| Abstract: | Summary We have investigated features for minimizing the inactivation of tyrosinase (E.C. 1.14.18.1) that is caused by immobilization on glass beads and Celite®. The degree of inactivation is dependent on the enzyme loading and the carrier's surface area. Addition of a sacrificial protein during the immobilization procedure offers a protective effect with increased residual activity on the basis of comparable enzyme loading. |
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