Inhibitory effects of myxothiazol and 2-n-heptyl-4-hydroxyquinoline-N-oxide on the auxiliary electron transport pathways of Rhodobacter capsulatus

The effects of various electron transport inhibitors upon the rates of reduction NO₃^-, dimethyl sulphoxide (DMSO) and N₂O in anaerobic suspensions of Rhodobacter capsulatus have been studied. A new method for the determination of the rates of reduction of these auxiliary oxidants in intact cells is presented, based on the proportionality observed between the concentration of oxidant and the duration of the electrochromic carotenoid bandshift. For NO₃^-and N₂O good agreement was found between rates of reduction determined using electrodes and those determined by the electrochromic method.Myxothiazol and antimycin A had no effect on the rates of reduction of NO₃^-and DMSO suggesting that the cytochrome b/c₁complex is not involved in electron transport to these oxidants. 2-n-heptyl-4-hydroxyquinoline-N-oxide (HOQNO) inhibited at two sites, one within the cytochrome b/c₁complex and the other on the nitrate reducing pathay, but had no effect on electron transport to N₂O or DMSO. In both intact cells and cell free extracts, HOQNO had no effect on the nitrate dependent re-oxidation of reduced methylviologen (MVH₂), a direct electron donor to nitrate reductase.Our data are consistent with a branch point for the auxiliary electron transport pathways at the level of the ubiquinone pool.Non-standard abbreviations HOQNO 2-n-heptyl-4-hydroxyquinoline-N-oxide - TMAO trimethylamine-N-oxide - DMSO dimethyl-sulphoxide - membrane potential - MVH₂ reduced methyl viologen