Abscisic Acid Translocation and Metabolism in Soybeans following Depodding and Petiole Girdling Treatments |
| |
Authors: | Setter T L Brun W A |
| |
Affiliation: | Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108. |
| |
Abstract: | It was found earlier that depodding and girdling treatments which obstruct translocation, result in increased leaf AbA levels and partial stomatal closure. In the present work (±) [2-14C]abscisic acid (AbA) was introduced into leaves and the mass, and radioactivity of AbA and AbA-metabolites were analyzed following translocation obstruction to determine whether the increased AbA was due to higher rates of synthesis, or lower rates of catabolism or export. The (±) [2-14C]AbA was introduced into soybean (Merr.) leaves by injection into the petiole region. AbA and AbA-metabolites (phaseic acid [PA], dihydrophaseic acid [DPA], AbA-conjugate, and an unknown metabolite) were separated with preparative high performance liquid chromatography. Methyl esters of AbA (free and that released after hydrolysis of AbA-conjugate), PA and DPA were determined with gas chromatography using electron capture detection. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|