| 水冬瓜种子薄切片的制备和染色技术 |
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| 引用本文： | 姚雅琴 胡东维. 水冬瓜种子薄切片的制备和染色技术[J]. 西北植物学报, 1992, 12(3): 249-252 |
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| 作者姓名： | 姚雅琴 胡东维 |
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| 作者单位： | 西北农业大学，西北农业大学，西北农业大学，西北农业大学 陕西杨陵 712100，陕西杨陵 712100，陕西杨陵 712100，陕西杨陵 712100 |
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| 摘 要： |
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| 关 键 词： | 薄片制备 种子结构 水冬瓜 染色法 |
A METHOD OF PREPARATION AND STAIN OF THIN SECTIONS FOR THE SEEDS OF IDESIA POLYCARPA VAR. VESTITA DIELS |
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| Yao Yaqin,Hu Dongwei,Jiang Xuanli and Yang Shuxing. A METHOD OF PREPARATION AND STAIN OF THIN SECTIONS FOR THE SEEDS OF IDESIA POLYCARPA VAR. VESTITA DIELS[J]. Acta Botanica Boreali-Occidentalia Sinica, 1992, 12(3): 249-252 |
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| Authors: | Yao Yaqin Hu Dongwei Jiang Xuanli Yang Shuxing |
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| Abstract: | A preparation and stain method of thin sections for small samples with hard, thick shells and soft internal tissues is dealt with in this paper. The results showed that the seeds of Idesia Polycarpa var. vestita Diels at different stages of development, after being immersed in concentrated sulfuric acid for different time to increase the permeability of the seed coats, can be easily prepared into thin sections with whole seed structure by usual method, and that staining in Methylene Blue and Eosin, various cell components can be respectively stained different colors. |
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| Keywords: | preparation of thin section stain method seed structure ldesia polycarpa var. vestita sulfuric acid immersing. |
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