Comparing protein abundance and mRNA expression levels on a genomic scale |
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| Authors: | Greenbaum Dov Colangelo Christopher Williams Kenneth Gerstein Mark |
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| Affiliation: | (1) Department of Genetics, Yale University, 06520-8114 New Haven, CT, USA;(2) Department of Molecular Biophysics and Biochemistry, Yale University, 06520-8114 New Haven, CT, USA;(3) HHMI Biopolymer Laboratory and W. M. Keck Foundation Biotechnology Resource Laboratory, Yale University, 06520-8114 New Haven, CT, USA;(4) Department of Computer Science, Yale University, 06520-8114 New Haven, CT, USA; |
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| Abstract: | Attempts to correlate protein abundance with mRNA expression levels have had variable success. We review the results of these comparisons, focusing on yeast. In the process, we survey experimental techniques for determining protein abundance, principally two-dimensional gel electrophoresis and mass-spectrometry. We also merge many of the available yeast protein-abundance datasets, using the resulting larger 'meta-dataset' to find correlations between protein and mRNA expression, both globally and within smaller categories. |
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