Lipid order and composition of synaptic membranes in experimental diabetes mellitus |
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| Authors: | Arshag D. Mooradian Frank Dickerson Thomas L. Smith |
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| Affiliation: | (1) The Medical Service, Department of Medicine, University of Arizona College of Medicine, 1821 East Elm Street, Tucson, AZ;(2) The Research Service, Department of Medicine, University of Arizona College of Medicine, 1821 East Elm Street, Tucson, AZ;(3) The Research Service Tucson VA Medical center, University of Arizona College of Medicine, Tucson, AZ;(4) The Research Service, Department of Pharmacology, University of Arizona College of Medicine, Tucson, AZ |
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| Abstract: | The effect of diabetes in rats on lipid composition and order of synaptosomal membranes (SM) was determined in streptozotocin-induced diabetic rats after 6 weeks of chronic hyperglycemia. The cholesterol content was slightly, but not significantly, higher in diabetic SM (0.287±0.042 vs. 0.209±0.061  mol/mg protein). The phospholipid concentration in diabetic SM was significantly increased (0.515±0.042 vs. 0.305±0.041 mol/mg protein;P<0.005). Neither the molar ratios of cholesterol to phospholipids in the SM nor the fatty acid composition of the SM was significantly altered with diabetes. Diabetes did not affect membrane order or the thermotropic transition temperature of the SM as determined fluorometrically. On the other hand, the SM of diabetic rats had significantly increased concentration of lipid peroxidation products, namely conjugated dienes (the calculated O.D./mol phospholipids was 11.56±1.83 in controls and 19.95 ±4.1 in diabetic ratsP<0.01). Despite the accumulation of lipid peroxidation byproducts in SM of diabetic rats the overall membrane order and the cholesterol to phospholipid molar ratio do not appear to be significantly altered. |
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| Keywords: | Synaptosome lipid order lipid composition conjugated dienes diabetes |
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