弥漫大B细胞淋巴瘤1号染色体的基因表达分析

目的:探讨弥漫大B细胞淋巴瘤(Diffuse Large B-Cell Lymphoma,DLBCL)中1号染色体基因表达情况。方法:采用激光显微切割技术分离临床DLBCL病人淋巴结标本中的淋巴细胞,提取淋巴细胞的mRNA并与表达谱芯片杂交,通过信号扫描、处理后获得表达基因杂交信号强度。每基因设11-20对探针。杂交信号与错配探针对比,扣除背景值后,使用Wilcoxon符号秩和检验选取与错配杂交信号有显著差异的基因作为分析结果(P=0.05)。然后随机选取四个检测到的基因,使用PCR方法检验基因芯片结果的可靠性。结果:成功地从快速冷冻保存的DLBCL标本中提取RNA。使用表达谱芯片进行研究,发现了共316条1号染色体编码的基因在DLBCL细胞中表达。根据胞内定位,基因功能和基因所属的代谢通路三种分类方法对所得基因进行分类分析。基因表达密度分析显示DLBCL中1号染色体上的基因表达情况与编码基因分布情况存在统计学差异。结论:使用表达谱芯片研究了DLBCL中1号染色体上的基因表达情况。

Transcriptional Analysis of Chromosome 1 in Diffuse Large B-Cell Lymphoma

Objective:To explore the profile of expressed genes encoded by chromosome 1 in lymph node of Diffuse Large B-Cell Lymphoma(DLBCL).Methods:Lymph node of DLBCL was quick-frozen in liquid nitrogen.Lymphoma cells were separated from frozen sections by LCM.The mRNA of the cells from the lymphoma lymph node was marked with biotin and hybridized with expression profile microarray,and the data of expressed genes were obtained.Initial bioinformatics analysis was performed.Results:Using expression profile microarray,316 genes encoded by chromosome 1 were found expressed in lym- phoma lymph node of DLBCL.The detected genes were classified by cellular component,molecular function and biological func- tion.Conclusions:The profile of expressed genes encoded by chromosome 1 in lymphoma lymph node of DLBCL had been ex- plored by using expression profile microarray.