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In vitro analysis of integrin expression in stem cells from bone marrow and cord blood during chondrogenic differentiation
Authors:Goessler Ulrich Reinhart  Bugert Peter  Bieback Karen  Stern-Straeter Jens  Bran Gregor  Sadick Haneen  Hörmann Karl  Riedel Frank
Affiliation:Department of Otolaryngology, Head and Neck Surgery, University Hospital Mannheim, University of Heidelberg, Mannheim, Germany;Institute of Transfusion Medicine and Immunology, Red Cross Blood Service of Baden-Württemberg/ Hessen gGmbH, Ruprecht Karls-University Heidelberg, Faculty of Clinical Medicine Mannheim, Germany
Abstract:The use of adult mesenchymal stem cells (MSC) in cartilage tissue engineering has been implemented in the field of regenerative medicine and offers new perspectives in the generation of transplants for reconstructive surgery. The extracellular matrix (ECM) plays a key role in modulating function and phenotype of the embedded cells and contains the integrins as adhesion receptors mediating cell-cell and cell-matrix interactions. In our study, characteristic changes in integrin expression during the course of chondrogenic differentiation of MSC from bone marrow and foetal cord blood were compared. MSC were isolated from bone marrow biopsies and cord blood. During cell culture, chondrogenic differentiation was performed. The expression of integrins and their signalling components were analysed with microarray and immunohistochemistry in freshly isolated MSC and after chondrogenic differentiation. The fibronectin-receptor (integrin a5b1) was expressed by undifferentiated MSC, expression rose during chondrogenic differentiation in both types of MSC. The components of the vitronectin/osteopontin-receptors (avb5) were not expressed by freshly isolated MSC, expression rose with ongoing differentiation. Receptors for collagens (a1b1, a2b1, a3b1) were weakly expressed by undifferentiated MSC and were activated during differentiation. As intracellular signalling components integrin linked kinase (ILK) and CD47 showed increasing expression with ongoing differentiation. For all integrins, no significant differences could be found in the two types of MSC. Integrin-mediated signalling seems to play an important role in the generation and maintenance of the chondrocytic phenotype during chondrogenic differentiation. Especially the receptors for fibronectin, vitronectin, osteopontin and collagens might be involved in the generation of the ECM. Intracellularly, their signals might be transduced by ILK and CD47. To fully harness the potential of these cells, future studies should be directed to ascertain their cellular and molecular characteristics for optimal identification, isolation and expansion.
Keywords:integrin    cartilage    tissue engineering    differentiation    extracellular matrix    mesenchymal stem cells    chondrogenic differentiation
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