A new DNA profiling system for cell line identification for use in cell banks in Japan |
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| Authors: | Masamitsu Honma Eiko Kataoka Kiyokata Ohnishi Tadao Ohno Masao Takeuchi Nobuo Nomura Hiroshi Mizusawa |
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| Affiliation: | (1) Division of Genetics and Mutagenesis, Cell Bank, National Institute of Hygienic Sciences, 1-18-1 Kami-yoga, Setagaya-ku, 158 Tokyo, Japan;(2) Cell Bank, Riken (RCB, The Institute of Physical and Chemical Research), 3-1-1 Koyadai, 305 Tsukuba Science City, Japan;(3) Institute for Fermentation, 2-17-85, Juso-Honmachi, Yodogawa-ku, Osaka, Japan;(4) Molecular Oncology Laboratory, Nippon Veterinary and Zootechnology College, Tokyo, Japan |
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| Abstract: | Summary Using the polymorphic DNA probes, ChdTC-15, ChdTC-114, pYNH24, and λTM-18, a DNA profiling system was developed that verified identities of individual cultured cell lines collected in the Japanese cell banks, JCRB, RCB, and IFO. These highly polymorphic DNA probes include both VNTR (Variable Number of Tandem Repeats) sequences and substantial lengths of unique regions. In the mixed probe system, several distinct bands from four to eight can be used for cell line identification. These bands were widely spread in a range of molecular sizes, and were stable and reproducible under stringent conditions of Southern blot hybridization. Because the DNA profile was specific for each individual human cell line, it is useful not only to authenticate many existing cultured cell lines but also to monitor their identity during propagation in a laboratory, and to confirm newly established lines as unique. |
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| Keywords: | human cell lines identification DNA fingerprinting DNA profiling allelogram |
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