pGFPGUSPlus, a new binary vector for gene expression studies and optimising transformation systems in plants |
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| Authors: | Claudia E. Vickers Peer M. Schenk Dongxue Li Philip M. Mullineaux Peter M. Gresshoff |
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| Affiliation: | (1) Department of Biological Sciences, The University of Essex, Wivenhoe Park, Colchester, C04 3SQ, England;(2) School of Integrative Biology, The University of Queensland, St Lucia, Brisbane, QLD 4072, Australia;(3) Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Queensland, St Lucia, Brisbane, QLD 4072, Australia |
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| Abstract: | A binary vector containing two reporter gene cassettes has been developed. This vector is ideal for optimising new plant transformation systems. Following optimisation, one of the reporter genes can be replaced with a gene of interest; the second can be used as a marker to confirm transgenic lines, and to estimate locus number and determine zygosity. This allows simple, efficient and economical screening for homozygous single-insert lines and azygous controls. |
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| Keywords: | Binary vector GFP GUSPlus Plant transformation Segregation analysis |
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