Biodegradation of methyl tert-butyl ether as a sole carbon source by aerobic granules cultivated in a sequencing batch reactor |
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Authors: | Li-Li Zhang R. Y. Zhu J. M. Chen W. M. Cai |
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Affiliation: | School of Biological and Environmental Engineering, Zhejiang University of Technology, No.6 District, Zhaohui, Hangzhou, 310032, China, llzhang@zjut.edu.cn. |
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Abstract: | Aerobic granules efficient at degrading methyl tert-butyl ether (MTBE) were successfully developed in a well-mixed sequencing batch reactor (SBR). Treatment efficiency of MTBE in the reactor during the stable operations exceeded 99.8%, and effluent MTBE was consistently below 800 mug/L. The specific MTBE degradation rate was observed to increase with increasing MTBE initial concentrations from 25 to 400 mg/L, peaked at 18.2 mg-MTBE/g-VSS h, and declined with further increases in MTBE concentration as substrate inhibition effects became significant. There was a good fit between these biodegradation data and the Haldane equation (R (2) = 0.976). Microbial community DNA profiling was carried out using denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction amplified 16S rDNA. The aerobic granule was found to contain a wide diversity of microorganisms. More than 70% similarity among the samples in the time period examined indicated a highly stable microbial community as the reactor reached the stable operation. |
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Keywords: | Aerobic granule MTBE Degradation rate DGGE Microbial community |
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