Diastereomers of nonionic oligonucleotide analogs. VI. Isolation of diastereomers of ethyl phosphotriesters of the octanucleotide d(GCCAAACA) by high performance affinity chromatography]期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Diastereomers of nonionic oligonucleotide analogs. VI. Isolation of diastereomers of ethyl phosphotriesters of the octanucleotide d(GCCAAACA) by high performance affinity chromatography]

Authors:	I A Vtorushina Iu A Gorbunov V F Zarytova N I Komarova A V Lebedev S G Lokhov A V Manaenko A N Siniakov I G Shishkina

Abstract:	Diastereomers of oligonucleotide ethyl phosphotriesters were separated by high-performance complementary (affinity) chromatography on a column with the immobilized complementary oligonucleotide. The elution buffer contained 0.18 M K2HPO4, pH 7.5, and 30% acetonitrile. The temperature of the separation was a few degrees lower than Tm of corresponding oligonucleotide complexes. The diastereomers separated completely or partially were: dGCC(Et)AAACA], dGCCA(Et)AACA], dGCAA(Et)ACA], dGCC(Et)A(Et)AACA], dGCC(Et)AA(Et)ACA], dGCCA(Et)A(Et)ACA], dGCC(Et)A(Et)A(Et)ACA].

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