Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona
curzoniae) |
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Authors: | Ke-xin Li Jia-ning Geng Jin Yang Yan-ming Zhang Song-nian Hu |
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Institution: | (1) Graduate School of the Chinese Academy of Sciences, Beijing, China;(2) Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Plateau Institute of Biology, The Chinese Academy of Sciences, No. 59 XiGuan Avenue, Xining, Qinghai, 810001, China;(3) Key Laboratory of Genome Information and Sciences, Beijing Institute of Genomics, The Chinese Academy of Sciences, No. 7 Beitucheng West Road, ChaoYang, Beijing, 100029, China |
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Abstract: | High quality jumping microsatellite libraries of the plateau pika (Ochotona curzoniae) were constructed; 231 out of the 288 clones contained microsatellite repeat motif and 120 pairs of primers were designed
accordingly. Polymorphism was assessed for 48 individuals. Only 13 microsatellite loci were polymorphic with high polymorphism
information content (PIC) ranging from 0.598 to 0.871, a condition probably resulting from individuals used for assessment
being very closely related. This degree of polymorphism, however, is sufficient to conduct parentage analysis. Expected and
observed heterozygosities ranged from 0.219 to 0.935 and 0.659 to 0.890, respectively. One locus was in linkage disequilibrium.
This information provides efficient tools to allow future parentage studies.
Ke-xin Li and Jia-ning Geng contributed equally to this paper. |
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Keywords: | Magnetic beads enrichment Microsatellite Parentage analysis Plateau pika Polymorphism |
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