| 利用同工酶和SDS-PAGE技术对一些兰属(Cymbidium)品种的分析(简报) |
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| 引用本文： | 叶庆生,文李,潘瑞炽.利用同工酶和SDS-PAGE技术对一些兰属(Cymbidium)品种的分析(简报)[J].热带亚热带植物学报,1999,7(4):337-341. |
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| 作者姓名： | 叶庆生 文李 潘瑞炽 |
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| 作者单位： | 华南师范大学生物系!广州５１０６３１ |
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| 摘 要： | 利用同工酶和ＳＤＳ－ＰＡＧＥ技术对兰属（Ｃｙｍｂｉｄｉｕｍ）５个种和２个变种的１１个品种进行了分类研究，酯酶和细胞色素氧化酶的同工酶共出现２４条带，其中２３条具有多态性；ＳＤＳ－ＰＡＧＥ有３０条带，其中２５条带为多态性。根据同工酶和ＳＤＳ－蛋白质标记进行聚类分析，生化水平与传统的形态学分类结果基本一致，但某些兰属品种的分类地位与传统的分类有一些差异。
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| 关 键 词： | 同工酶 SDS－PAGE 兰属 聚类分析 |
GERMPLASM ANALYSIS IN SOME CYMBIDIUM CULTIVARS USING ISOENZYME AND SDS-PAGE |
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| Ye Qingsheng,Wen Li,Pan Ruichi.GERMPLASM ANALYSIS IN SOME CYMBIDIUM CULTIVARS USING ISOENZYME AND SDS-PAGE[J].Journal of Tropical and Subtropical Botany,1999,7(4):337-341. |
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| Authors: | Ye Qingsheng Wen Li Pan Ruichi |
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| Abstract: | Isoenzyme and SDS-PAGE techniques were used to analyze 11 cultivars of genusCymbidium. Among 24 bands of esterase and cytochrome oxidase, 23 were polymorphic,and 25 of 30 bands produced in SDS-PAGE were polymorphic. Based on the clusteranalysis of these markers, biochemical results were consistent with those of traditionaltaxonomic study, except that the systematic status for several Cymbidium cultivars weredifferent from the mophological classification. |
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| Keywords: | Isoenzyme SDS-PAGE Cymbidium Cluster analysis |
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