Crystallization and preliminary crystallographic analysis of allograft inflammatory factor 1 |
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Authors: | Li Shentao Liao Xiaoping Chen Zhangliu Bartlam Mark |
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Affiliation: | The Capital University of Medical Science, Beijing, 100054, People's Republic of China. lishentao@sina.com |
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Abstract: | Allograft inflammatory factor-1 (AIF-1) is a 17-kDa IFN-gamma inducible Ca2+-binding EF-hand protein involved in immune dysfunction and smooth muscle cell activation. AIF-1 was solubly expressed in E. coli and purified. Crystals of AIF-1 were grown at 291 K using PEG-8000 as precipitant. Diffraction by the AIF-1 crystal extends to 3.3 A resolution, and the crystal belongs to the space group P4(3) with unit cell parameters a=b=73.4, c=49.1 A. |
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