Improved basal medium for Y-1 mouse adrenal cortex tumor cells in culture |
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Authors: | Lucian J. Cuprak Carol J. Lammi Janet I. Crane |
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Affiliation: | (1) Medical Research Service, Veterans Administration Center, 04330 Togus, Maine;(2) Department of Biochemistry, University of Maine, Orono, Maine |
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Abstract: | Summary An improved basal medium is presented that requires only minimal supplementation with dialyzed fetal bovine serum or bovine serum albumin and fetuin to be comparable to Ham's F-10, which requires 15% horse serum (HS) and 2.5% fetal bovine serum (FBS) for the growth and function of Y-1, mouse adrenal cortex tumor, cells. Cell monolayers maintained for up to 2 weeks without any protein supplementation have retained their steroid response to ACTH. The medium differs from Ham's F-10 in its buffer composition and higher calcium-ion concentration. This medium should be a useful adjunct to studies pertaining to steroid and lipid intermediary metabolism, the retention of a specialized physiological function in a chemically defined medium, and the mechanism of hormonal response. Supported by the Medical Research Service of the Veterans Administration. |
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Keywords: | adrenal cell cultures medium buffer calcium |
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