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Bioavailability and metabolism of fucoxanthin in rats: structural characterization of metabolites by LC-MS (APCI) |
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| Authors: | Ravi Kumar Sangeetha Narayan Bhaskar Sounder Divakar Vallikannan Baskaran |
| Affiliation: | 1. Department of Biochemistry and Nutrition, Central Food Technological Research Institute, CSIR, Mysore, 570 020, India 2. Department of Meat, Fish and Poultry Technology, Central Food Technological Research Institute, CSIR, Mysore, 570 020, India 3. Department of Fermentation Technology and Bioengineering, Central Food Technological Research Institute, CSIR, Mysore, 570 020, India |
| Abstract: | This study reports bioavailability and metabolism of fucoxanthin (FUCO) from brown algae Padina tetrastromatica in rats. Rats were divided into two groups (n = 25/group). Group one was fed basal diet (control) while the group two received retinol deficient diet (RD group) for 8 weeks. After confirmed RD in blood (0.53 μmol/l), rats were further sub-grouped (n = 5/sub group), intubated a dose of FUCO (0.83 μmol) and killed after 0, 2, 4, 6 and 8 h. The plasma levels (area under curve/8 h) of FUCO (fucoxanthinol (FUOH) + amarouciaxanthin (AAx)) was 2.93 (RD group) and 2.74 pmol/dl (control), respectively. No newly formed retinol was detected in RD rats intubated with FUCO. Besides FUOH (m/z 617 (M+H)+) and AAx (m/z 617 (M+H?)+), other deacetylated, hydrolyzed and demethylated metabolites of bearing molecular mass at m/z 600.6 (FUOH–H2O), m/z 597 (AAx–H2O), m/z 579 (AAx–2H2O+1), m/z 551 (AAx–2H2O–2CH3+2) and m/z 523 (AAx–2H2O–4CH3+4) were also detected in plasma and liver by LC-MS (APCI). Although biological functions of FUCO metabolites need thorough investigation, this is the first detailed report on FUCO metabolites in rats. |
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