A novel extracellular esterase from Bacillus subtilis and its conversion to a monoacylglycerol hydrolase. |
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| Authors: | T Eggert G Pencreac'h I Douchet R Verger K E Jaeger |
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| Affiliation: | Lehrstuhl Biologie der Mikroorganismen, Ruhr-Universit?t Bochum, Germany. |
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| Abstract: | A novel gene lipB, which encodes an extracellular lipolytic enzyme, was identified in the Bacillus subtilis genomic DNA sequence. We have cloned and overexpressed lipB in B. subtilis and Escherichia coli and have also purified the enzyme from a B. subtilis culture supernatant to electrophoretic homogeneity. Four different lipase assays were used to determine its catalytic activity: pH-stat, spectrophotometry, fluorimetry and the monomolecular film technique. LipB preferentially hydrolysed triacylglycerol-esters and p-nitrophenyl-esters of fatty acids with short chain lengths of
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