Cloning and Characterization of High-CO2-Specific cDNAs from a Marine Microalga, Chlorococcum littorale, and Effect of CO2 Concentration and Iron Deficiency on the Gene Expression |
| |
Authors: | Sasaki, Takayuki Kurano, Norihide Miyachi, Shigetoh |
| |
Affiliation: | 1 Marine Biotechnology Institute, Kamaishi Laboratories 3-75-1 Heita, Kamaishi City, Iwate, 026-0001 Japan 2 Marine Biotechnology Institute Head Office, 1-28-10 Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan |
| |
Abstract: | Two cDNA clones exclusively induced under an extremely high-CO2concentration (20%) were isolated from Chlorococcum littoraleby differential screening and named HCR (high-CO2 response)1 and 2, respectively. The amino acid sequence of the proteinencoded by HCR2 exhibited homology to the gp91-phox protein,a critical component of a human phagocyte oxidoreductase, andto the yeast ferric reductases, Saccharomyces cerevisiae FRE1and FRE2 and Schizosaccharomyces pombe Frpl. The induction ofboth HCR mRNAs required extremely high-CO2 conditions and irondeficiency, being suppressed under air conditions and by ironsufficiency, suggesting that the expression of these two HCRgenes required extremely high-CO2 conditions and iron deficiencyin combination. The HCR2 protein was detected in the membranefractions of cells grown under conditions which would favorthe induction of HCR2-mRNA and the protein level was loweredwhen the cells were transferred from iron deficient to 10 µMFeSO4 conditions (with 20% CO2). (Received September 10, 1997; Accepted November 14, 1997) |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|