A new method for fish chromosome preparation |
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| Authors: | Z Fan D P Fox † |
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| Institution: | Heilongjiang Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 20 Huaxing 5th Street, Harbin, 150076, P. R. China;Department of Genetics, University of Aberdeen, 2 Tillydrone Avenue, Aberdeen AB9 2TN, U.K. |
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| Abstract: | A new method for the preparation of fish chromosomes from abdominal cavity fluid has been developed. Cells were collected from fish abdominal cavity fluid after an in vivo PHA treatment, and cultured for a short time in medium with colchicine. After 30 min hypotonic treatment for marine fish and 35 min for freshwater fish, slides were prepared by the conventional air-drying method. The advantages ofthe method are: (1) it is technically simple; (2) it produces a reasonably high mitotic index; (3) chromosome spreading is good (4) there is very little cell breakage. Using this method, the chromosomes ofrainbow trout (2n=62); cod (2n=4546) and plaice (2n=46,47 and 48) were investigated. |
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| Keywords: | chromosome preparation rainbow trout cod plaice cell culture |
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