Inactivation of nitrate reductase from wheat and rice leaves |
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Authors: | Chodavarapu S. Ramarao Manohar S. Naik |
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Affiliation: | Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110012, India |
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Abstract: | In fresh leaves, the inactivation of nitrate reductase was rapid at high temperatures as compared to low temperatures. In leaves subjected to freeze-thaw treatment, the loss of enzyme activity was extremely rapid particularly at high temperatures. Pre-incubation with NADH not only protected the enzyme against inactivation, but also stimulated its activity. In dialysed extracts of rice leaves, NADH alone offered some protection while nitrate alone did not protect the enzyme from inactivation. Addition of both NADH and nitrate during pre-incubation enhanced the enzyme activity considerably. It is suggested that stimulation of nitrate reduction by NADH and nitrate may be of physiological significance to the plant, in the sense that in the presence of sufficient supplies of reluctant and nitrate, the process of nitrate assimilation would be accelerated. |
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Keywords: | Gramineae rice wheat nitrate reductase inactivating factor NADH. |
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