Hydrodynamic properties of mushroom tyrosinase |
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| Authors: | Rakesh C Sharma Rashid Ali |
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| Institution: | Department of Biochemistry, J. N. Medical College, Aligarh Muslim University, Aligarh 202001, India |
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| Abstract: | The hydrodynamic properties of mushroom tyrosinase were determined at pH 6.5 using a Sephadex G-200 column. From the comparison of its gel-filtration behaviour with those of standard proteins, the following parameters were calculated: MW (122 500 ± 1%), Stokes' radius (42.75 × 10?8 cm2/sec), diffusion coefficient (5.048 × 10?7 cm2/sec) and frictional ratio (1.26). These values suggest a globular conformation of this enzyme. |
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| Keywords: | Agaricaceae tyrosinase mushroom hydrodynamic properties enzyme |
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