Ultrastructural immunocytochemical localization of neuron-specific enolase in parafollicular cells of the rat thyroid |
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Authors: | M Zabel H Schäfer |
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Institution: | (1) Department of Histology and Embryology, Academy of Medicine, ul. wiecickiego 6, PL-60781 Pozna , Poland;(2) Institute of Pathology, University of Hamburg, Martinistrasse 52, D-2000 Hamburg 20, Germany |
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Abstract: | Summary Using pre- and post-embedding procedures, neuron-specific enolase and calcitonin were localized in rat thyroid parafollicular cells by light and electron microscopy. Peroxidase-antiperoxidase (PAP), biotin-avidin (ABC) and protein A — colloidal gold techniques were used. In paraffin sections neuron-specific enolase was demonstrated in all calcitonin-storing parafollicular cells in rats aging 1 to 180 days. The post-embedding procedure failed to detect neuron-specific enolase in ultrathin sections, but the enzyme could be demonstrated using a preembedding procedure. Neuron-specific enolase was localized exclusively within the cytosol of parafollicular cells, while calcitonin was localized within secretory granules applying either post- or pre-embedding incubation techniques.Supported by Sonderforschungsbereich 232 |
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