Screening and production of erythritol by newly isolated osmophilic yeast-like fungi |
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Authors: | Shie-Jea Lin Chiou-Yen Wen Jian-Ching Liau Wen-Shen Chu |
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Institution: | Food Industry Research and Development Institute, PO Box 246, Hsinchu 30099, Taiwan, ROC |
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Abstract: | Twenty-eight erythritol-producing strains were isolated from pollen, honey and high sugar food samples collected in Taiwan. Amongst these, six strains (166-2, 262-1, 278-3, 440, 441 and 442) were high erythritol-producers with a yield higher than 30% for 30% glucose. The erythritol productivity of these strains ranged from 90.9 to 116.4 g l?1. 1H- and 13C-NMR analyses confirmed that the fermentation product was erythritol. The results of morphological and physiological studies indicate that strains 166-2, 262-1, 278-3, 440, and 442 may be members of the genus Moniliella. More studies are required to determine the taxonomic position of strain 441. The use of a medium containing 30% glucose and 1% yeast extract gave the highest erythritol productivity. On batch fermentation in a 5-l fermentor using strain 166-2, a maximal erythritol productivity of 111.0 g l?1 was obtained after cultivation for 144 h. |
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Keywords: | Erythritol Yeast-like fungi Screening |
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